Optimization of potting mixture for hardening of in vitro raised plants of Tylophora indica to ensure high survival percentage

Micropropagation is an important technique for rapid and large-scale multiplication of selected plants. The type and concentration of plant growth regulators and physiological status of the explant have profound effect on the efficacy of plant regeneration. However, success of any micropropagation protocol depends upon the percentage survival and successful establishment of plants from in vitro to ex vitro conditions. In the present study, a successful attempt has been made to acclimatize the tissue culture raised plants of Tylophora indica using various potting mixes of soil, vermicompost and biofertilizers (Azotobacter and Pseudomonas) in different combinations. Among all the combinations tried, soil: vermicompost: Azotobacter (N2 fixer): Pseudomonas (phosphate solubilizers) (1:1:1:1) showed the highest percentage survival (92%) upon transplantation of plants to the field conditions followed by soil: vermicompost (1:1) with 88% survival. Different parameters like plant height from the base of stem in the polybags, number of phytosynthetically active leaves/plant and mortality rate were also monitored periodically.